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primary antibodies against collagen type ii col ii  (Proteintech)


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    Proteintech primary antibodies against collagen type ii col ii
    Primary Antibodies Against Collagen Type Ii Col Ii, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+collagen+type+ii+col+ii/SGCG+Antibody/pmc10840349-87-29-37
    Average 96 stars, based on 398 article reviews
    primary antibodies against collagen type ii col ii - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: Injectable silk fibroin peptide nanofiber hydrogel composite scaffolds for cartilage regeneration
    Article Snippet: .. Protein samples of different molecular weights were separated via SDS‒PAGE (Epizyme, China), then transferred to a nitrocellulose membranes and blocked with 5 % skim milk powder for 1 h. Primary antibodies against collagen type II (Col-II) (1:1000, 28459-1-AP,Proteintech, China) and recombinant sex determining region Y box protein 9 (Sox9) (1:1000, 67439-1-Ig, Proteintech, China) were used to specifically label the target proteins. .. The corresponding protein signals were then labelled with secondary antibodies and developed via chemiluminescence (UVITEC, UK) with an enhanced chemiluminescence ECL kit (SQ101, Epizyme, China).



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    primary antibodies against collagen type ii col ii  (Proteintech)
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    primary antibodies against col ii  (Proteintech)
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    Protective effects of RUT against IL-1β-induced inflammation and extracellular matrix degradation in mouse chondrocytes. The cells were exposed to IL-1β (5 ng/ml) with/without RUT (1, 2.5, 5 and 10 μ M) for 24 h. (A and B) The protein expression levels of MMP3, MMP13, IL-1β, COX2, IL-6, TNF-α, SOX9, <t>COL</t> <t>II</t> and aggrecan were determined using western blot analysis. (C-G) Quantification analysis of the results of western blotting. (D and E) Relative mRNA expression levels of IL-6 and TNF-α in chondrocytes stimulated with IL-1β (5 ng/ml) and treated with or without RUT (1, 2.5, 5 and 10 μ M) for 24 h. The values are presented as the mean ± SD of three independent experiments. # P<0.05 vs. control group; ** P<0.01, *** P<0.001 and **** P<0.0001 vs. IL-1β group. (H) The protein expression of COL II was detected using immunofluorescence following treatment of the cells with IL-1β (5 ng/ml) with/without RUT (10 μ M) for 24 h. IL-1β, interleukin 1 β; RUT, rutaecarpine; MMP, matrix metalloproteinase; COX2, cyclooxygenase 2; SOX9, SRY-box transcription factor 9; COL II, collagen type II.
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    primary antibody against col ii  (Proteintech)
    96
    Proteintech primary antibody against col ii
    Protective effects of RUT against IL-1β-induced inflammation and extracellular matrix degradation in mouse chondrocytes. The cells were exposed to IL-1β (5 ng/ml) with/without RUT (1, 2.5, 5 and 10 μ M) for 24 h. (A and B) The protein expression levels of MMP3, MMP13, IL-1β, COX2, IL-6, TNF-α, SOX9, <t>COL</t> <t>II</t> and aggrecan were determined using western blot analysis. (C-G) Quantification analysis of the results of western blotting. (D and E) Relative mRNA expression levels of IL-6 and TNF-α in chondrocytes stimulated with IL-1β (5 ng/ml) and treated with or without RUT (1, 2.5, 5 and 10 μ M) for 24 h. The values are presented as the mean ± SD of three independent experiments. # P<0.05 vs. control group; ** P<0.01, *** P<0.001 and **** P<0.0001 vs. IL-1β group. (H) The protein expression of COL II was detected using immunofluorescence following treatment of the cells with IL-1β (5 ng/ml) with/without RUT (10 μ M) for 24 h. IL-1β, interleukin 1 β; RUT, rutaecarpine; MMP, matrix metalloproteinase; COX2, cyclooxygenase 2; SOX9, SRY-box transcription factor 9; COL II, collagen type II.
    Primary Antibody Against Col Ii, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+collagen+type+ii+col+ii/Collagen+Type+II+Antibody/pmc09462983-81-0-4
    Average 96 stars, based on 1 article reviews
    primary antibody against col ii - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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    Protective effects of RUT against IL-1β-induced inflammation and extracellular matrix degradation in mouse chondrocytes. The cells were exposed to IL-1β (5 ng/ml) with/without RUT (1, 2.5, 5 and 10 μ M) for 24 h. (A and B) The protein expression levels of MMP3, MMP13, IL-1β, COX2, IL-6, TNF-α, SOX9, COL II and aggrecan were determined using western blot analysis. (C-G) Quantification analysis of the results of western blotting. (D and E) Relative mRNA expression levels of IL-6 and TNF-α in chondrocytes stimulated with IL-1β (5 ng/ml) and treated with or without RUT (1, 2.5, 5 and 10 μ M) for 24 h. The values are presented as the mean ± SD of three independent experiments. # P<0.05 vs. control group; ** P<0.01, *** P<0.001 and **** P<0.0001 vs. IL-1β group. (H) The protein expression of COL II was detected using immunofluorescence following treatment of the cells with IL-1β (5 ng/ml) with/without RUT (10 μ M) for 24 h. IL-1β, interleukin 1 β; RUT, rutaecarpine; MMP, matrix metalloproteinase; COX2, cyclooxygenase 2; SOX9, SRY-box transcription factor 9; COL II, collagen type II.

    Journal: International Journal of Molecular Medicine

    Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3

    doi: 10.3892/ijmm.2023.5300

    Figure Lengend Snippet: Protective effects of RUT against IL-1β-induced inflammation and extracellular matrix degradation in mouse chondrocytes. The cells were exposed to IL-1β (5 ng/ml) with/without RUT (1, 2.5, 5 and 10 μ M) for 24 h. (A and B) The protein expression levels of MMP3, MMP13, IL-1β, COX2, IL-6, TNF-α, SOX9, COL II and aggrecan were determined using western blot analysis. (C-G) Quantification analysis of the results of western blotting. (D and E) Relative mRNA expression levels of IL-6 and TNF-α in chondrocytes stimulated with IL-1β (5 ng/ml) and treated with or without RUT (1, 2.5, 5 and 10 μ M) for 24 h. The values are presented as the mean ± SD of three independent experiments. # P<0.05 vs. control group; ** P<0.01, *** P<0.001 and **** P<0.0001 vs. IL-1β group. (H) The protein expression of COL II was detected using immunofluorescence following treatment of the cells with IL-1β (5 ng/ml) with/without RUT (10 μ M) for 24 h. IL-1β, interleukin 1 β; RUT, rutaecarpine; MMP, matrix metalloproteinase; COX2, cyclooxygenase 2; SOX9, SRY-box transcription factor 9; COL II, collagen type II.

    Article Snippet: Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.

    Techniques: Expressing, Western Blot, Control, Immunofluorescence

    The beneficial effects of RUT on IL-1β-stimulated chondrocytes were attenuated following integrin αVβ3 knockdown. (A and B) Relative mRNA levels of ItgαV and Itgβ3 in chondrocytes exposed to 5 ng/ml IL-1β with/without RUT (1, 2.5, 5 and 10 μ M) for 24 h. The knockdown efficiency of ItgαV and Itgβ3 siRNA transfection was verified using RT-qPCR. (C-E and H-M) The expression levels of inflammation and extracellular matrix degradation-related proteins were determined using western blot analysis; the outcomes of the chondrocytes were quantified in the presence/absence of 5 ng/ml IL-1β, RUT (10 μ M) and ItgαVβ3 for 24 h. (F and G, and N-S) Western blot analysis and quantification analysis were used to measure the levels of PI3K/Akt/NF-κB and MAPK-related signalling proteins in IL-1β (5 ng/ml)-stimulated chondrocytes for 30 min following transfection with/without ItgαVβ3 siRNA and RUT (10 μ M) for 24 h. Data are presented as the mean ± SD of three independent experiments. # P<0.05 vs. control group; * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001 vs. IL-1β group; ns, no significant difference (P>0.05). IL-1β, interleukin 1 β; RUT, rutaecarpine; MMP, matrix metalloproteinase; COX2, cyclooxygenase 2; SOX9, SRY-box transcription factor 9; PI3K, phosphoinositide-3-kinase; COL II, collagen type II; AGG, aggrecan.

    Journal: International Journal of Molecular Medicine

    Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3

    doi: 10.3892/ijmm.2023.5300

    Figure Lengend Snippet: The beneficial effects of RUT on IL-1β-stimulated chondrocytes were attenuated following integrin αVβ3 knockdown. (A and B) Relative mRNA levels of ItgαV and Itgβ3 in chondrocytes exposed to 5 ng/ml IL-1β with/without RUT (1, 2.5, 5 and 10 μ M) for 24 h. The knockdown efficiency of ItgαV and Itgβ3 siRNA transfection was verified using RT-qPCR. (C-E and H-M) The expression levels of inflammation and extracellular matrix degradation-related proteins were determined using western blot analysis; the outcomes of the chondrocytes were quantified in the presence/absence of 5 ng/ml IL-1β, RUT (10 μ M) and ItgαVβ3 for 24 h. (F and G, and N-S) Western blot analysis and quantification analysis were used to measure the levels of PI3K/Akt/NF-κB and MAPK-related signalling proteins in IL-1β (5 ng/ml)-stimulated chondrocytes for 30 min following transfection with/without ItgαVβ3 siRNA and RUT (10 μ M) for 24 h. Data are presented as the mean ± SD of three independent experiments. # P<0.05 vs. control group; * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001 vs. IL-1β group; ns, no significant difference (P>0.05). IL-1β, interleukin 1 β; RUT, rutaecarpine; MMP, matrix metalloproteinase; COX2, cyclooxygenase 2; SOX9, SRY-box transcription factor 9; PI3K, phosphoinositide-3-kinase; COL II, collagen type II; AGG, aggrecan.

    Article Snippet: Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.

    Techniques: Knockdown, Transfection, Quantitative RT-PCR, Expressing, Western Blot, Control